In the Southern blot technique, which of the following is NOT required?

Name: In the Southern blot technique, which of the following is NOT required?
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The addition of heat-stable DNA polymerase to amplify DNA is not required in Southern blotting. Instead, the technique utilizes restriction enzymes to digest DNA, electrophoresis to separate fragments, transfer of the DNA to nitrocellulose, and addition of a labeled probe to identify the gene of interest.

In the Southern Blot Technique, Which of the Following Is