Which of the following pairs is mismatched?
A) methylene blue - simple stain
B) nigrosin - negative stain
C) basic dye - negative stain
D) acidic dye - capsule stain
E) crystal violet - simple stain

Question

Quizplus · Accepted Answer

Basic dyes are used in positive staining techniques (such as simple stain, Gram stain, etc.) where the dye binds to the cells and impart color while negative staining is used to visualize cells that are too delicate to be heat-fixed or cells that cannot be stained by other methods. In negative staining, acidic dyes are used. Therefore, the pair of basic dye-negative stain is mismatched.

Which of the Following Pairs Is Mismatched