What Led to the Loss of Dignity and Place in Society

What led to the loss of dignity and place in society of two-spirit people among the Native Americans?

A) The Native Americans disliked these individuals as they often expressed their gender through their sexual practices.
B) European colonization and persecution by the church to eradicate these individuals resulted in the two-spirit community being viewed as perverted, untraditional, and untrustworthy.
C) The French took over the Native Americans to specifically target homosexuals and two-spirit people because they functioned against the laws of nature.
D) The Russian invasion aimed to bring about a religious reformation of the Native Americans, and all those who opposed this change were persecuted.