The specificity constant is equal to A) [E_t][S] / v₀. B) K_m/ v₀. C) k_cat/ K_m. D) k_cat/ [E_t].

Question

Quizplus · Accepted Answer

The specificity constant (kcat/Km) is the rate constant for the reaction between the enzyme and substrate and represents the efficiency of an enzyme in converting substrate to product. It is calculated as kcat (catalytic constant or turnover number, number of substrate molecules converted to product per unit time by one enzyme molecule) divided by Km (substrate concentration at which the rate of reaction is half of Vmax). Therefore, option C is the correct formula for the specificity constant.

The Specificity Constant Is Equal to