Calculate the purification of the target protein when there is a 30% decrease in activity and a 55% decrease in total protein after centrifugation.
A) 0.55-fold
B) 0.64-fold
C) 1.6-fold
D) 1.8-fold

Question

Quizplus · Accepted Answer

The purification factor is the ratio of the target protein activity before and after a purification step. In this case, there is a 30% decrease in activity and a 55% decrease in total protein after the centrifugation step.

Therefore, the purification factor can be calculated as:

Purification Factor = (Activity after centrifugation / Activity before centrifugation) / (Total protein after centrifugation / Total protein before centrifugation)

Substituting the values given:

Purification Factor = (0.7 / 1) / (0.45 / 1) = 1.56

This means that the target protein has been purified 1.56-fold.

Therefore, the best choice is C) 1.6-fold, as it is the closest to the calculated value of the purification factor.

Calculate the Purification of the Target Protein When There Is