The advantage of using a native PAGE gel compared with an SDS-PAGE gel is that the native PAGE gel
A) separates proteins only based on molar mass.
B) gives information on the charge or conformation of the protein.
C) results in a better separation of small proteins.
D) increases the resolution of large and small proteins.

Question

Quizplus · Accepted Answer

A native PAGE gel can separate proteins based on their charge, conformation, and shape, in addition to their molar mass, whereas an SDS-PAGE gel separates proteins solely based on their molar mass. Therefore, using a native PAGE gel can provide additional information about the properties of a protein.

The Advantage of Using a Native PAGE Gel Compared with an SDS-PAGE