In isoelectric focusing,
A) negatively charged proteins migrate toward the cathode.
B) negatively charged proteins migrate toward the anode.
C) small proteins migrate the fastest.
D) large proteins migrate the fastest.

Question

Quizplus · Accepted Answer

Isoelectric focusing separates proteins based on their isoelectric point (pI), which is the pH at which the protein has no net charge. Proteins with a higher pI are more positively charged and will migrate towards the cathode (negatively charged electrode), while proteins with a lower pI are more negatively charged and will migrate towards the anode (positively charged electrode). Therefore, negatively charged proteins migrate towards the cathode. Additionally, smaller proteins will migrate faster than larger proteins as they encounter less resistance during migration.

In Isoelectric Focusing