A polypeptide was digested by trypsin and chymotrypsin. Use the following information to determine the polypeptide sequence.
A) ECFGMREDWKYLM
B) LMYKWMGFCEDER
C) LMYKWDERMGFCE
D) CELMYKWDERMGF

Question

A polypeptide was digested by trypsin and chymotrypsin. Use the following information to determine the polypeptide sequence. 
A) ECFGMREDWKYLM 
B) LMYKWMGFCEDER 
C) LMYKWDERMGFCE 
D) CELMYKWDERMGF

Quizplus · Accepted Answer

The amino acid sequence obtained from trypsin is LMYKWDER and the sequence obtained from chymotrypsin is MGFCE. By overlapping these two sequences, we get the final sequence LMYKWDERMGFCE. Choice C is the only option that matches this sequence.

A Polypeptide Was Digested by Trypsin and Chymotrypsin