During solid-phase peptide synthesis,dicyclohexylcarbodiimide is used
A) as a blocking agent.
B) as an activating agent.
C) to cleave the peptide from the resin.
D) to couple amino acids during the synthesis.

Question

Quizplus · Accepted Answer

Dicyclohexylcarbodiimide (DCC) is used as an activating agent during solid-phase peptide synthesis. It works by activating the carboxylic acid group of the incoming amino acid, allowing it to react with the amino group on the growing peptide chain. DCC forms an activated ester intermediate, which then reacts with the amino group. This coupling reaction results in the formation of a peptide bond between the incoming amino acid and the growing peptide chain.

During Solid-Phase Peptide Synthesis,dicyclohexylcarbodiimide Is Used