In the production of recombinant DNA,which enzyme links matching cohesive ends using covalent interactions?
A) DNA methylase
B) restriction endonucleases
C) reverse transcriptase
D) DNA ligase

Question

Quizplus · Accepted Answer

DNA ligase is the enzyme that links matching cohesive ends using covalent interactions during the production of recombinant DNA. It catalyzes the formation of phosphodiester bonds between the 3' hydroxyl group of one nucleotide and the 5' phosphate group of another nucleotide. This process results in the restoration of the original DNA backbone and the formation of a continuous DNA strand.

In the Production of Recombinant DNA,which Enzyme Links Matching Cohesive