When a gene sequence is cloned using mRNA,which enzyme is used to seal the single-strand gaps left behind in the second strand of DNA?
A) DNA methylase
B) restriction endonucleases
C) reverse transcriptase
D) DNA ligase

Question

Quizplus · Accepted Answer

DNA ligase is the enzyme used to seal single-strand gaps left behind in the second strand of DNA during mRNA cloning. It catalyzes the formation of phosphodiester bonds between adjacent nucleotides in DNA strands. The other options, DNA methylase, restriction endonucleases, and reverse transcriptase, do not have a role in sealing single-strand gaps, but rather in other aspects of gene cloning and manipulation.

When a Gene Sequence Is Cloned Using MRNA,which Enzyme Is