What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
A) Transform bacteria with a recombinant DNA molecule.Cut the plasmid DNA using restriction enzymes.Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.Extract plasmid DNA from bacterial cells.Use ligase to seal plasmid DNA to nonplasmid DNA.
B) Cut the plasmid DNA using restriction enzymes.Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.Use ligase to seal plasmid DNA to nonplasmid DNA.Extract plasmid DNA from bacterial cells.Transform bacteria with a recombinant DNA molecule.
C) Extract plasmid DNA from bacterial cells.Cut the plasmid DNA using restriction enzymes.Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.Use ligase to seal plasmid DNA to nonplasmid DNA.Transform bacteria with a recombinant DNA molecule.
D) Extract plasmid DNA from bacterial cells.Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.Use ligase to seal plasmid DNA to nonplasmid DNA.Transform bacteria with a recombinant DNA molecule.Cut the plasmid DNA using restriction enzymes.
Correct Answer:
Verified
Q49: In analyzing the number of different bases
Q51: Q52: You briefly expose bacteria undergoing DNA replication Q53: What is the basis for the difference Q53: Which of the following sequences in double-stranded Q56: For a science fair project,two students decided Q58: A eukaryotic gene has "sticky ends" produced Q59: Why is it so important to be Q60: In the late 1950s,Meselson and Stahl grew Q62: The elongation of the leading strand during![]()
Unlock this Answer For Free Now!
View this answer and more for free by performing one of the following actions
Scan the QR code to install the App and get 2 free unlocks
Unlock quizzes for free by uploading documents