Changing a Single Amino Acid in a Protein Consisting of 433

Changing a single amino acid in a protein consisting of 433 amino acids would

A) always alter the primary structure of the protein but never alter its tertiary structure or function.
B) always alter the primary structure of the protein and sometimes alter its tertiary structure or function.
C) always alter the primary and tertiary structures of the protein but never alter its function.
D) sometimes alter the primary and tertiary structures of the protein but always alter its function.