Cell biologists employ targeted fluorescent dyes or modified fluorescent proteins in both standard fluorescence microscopy and confocal microscopy to observe specific details in the cell.Even though fluorescence permits better visualization, the resolving power is essentially the same as that of a standard light microscope because the resolving power of a fluorescent microscope is still limited by the __________ of visible light.

Question

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The resolving power of a microscope is limited by wavelength. This means that the smallest distance between two points that can be distinguished is proportional to the wavelength of the light being used. In the case of fluorescence microscopy, although targeted fluorescent dyes or modified fluorescent proteins allow for better visualization, the resolving power is still limited by the wavelength of visible light.

Cell Biologists Employ Targeted Fluorescent Dyes or Modified Fluorescent Proteins