Which of the following methods would be the most suitable to assess levels of expression of your target protein in different cell types?

Question

Quizplus · Accepted Answer

Western blot analysis is the most suitable method for determining the levels of expression of a specific protein in different cell types. It allows for the detection and quantification of a specific protein using specific antibodies, making it a reliable and accurate method for measuring protein expression. Gel-filtration and ion-exchange chromatography are separation techniques that can be used to purify proteins, but they do not provide information about levels of expression. Gel electrophoresis can be used to separate proteins according to their size, but it does not provide information about levels of expression unless combined with a detection method such as western blot analysis.

Which of the Following Methods Would Be the Most Suitable