You wish to produce a human enzyme, protein A, by introducing its gene into bacteria.The genetically engineered bacteria make large amounts of protein A, but it is in the form of an insoluble aggregate with no enzymatic activity.Which of the following procedures might help you to obtain soluble, enzymatically active protein? Select all options that may be useful.Explain your reasoning.
A.Make the bacteria synthesize protein A in smaller amounts.
B.Dissolve the protein aggregate in urea, then dilute the solution and gradually remove the urea.
C.Treat the insoluble aggregate with a protease.
D.Make the bacteria overproduce chaperone proteins in addition to protein A.
E.Heat the protein aggregate to denature all proteins, then cool the mixture.

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The Answer of You wish to produce a human enzyme, protein A, by introducing its gene into bacteria.The genetically engineered bacteria make large amounts of protein A, but it is in the form of an insoluble aggregate with no enzymatic activity.Which of the following procedures might help you to obtain soluble, enzymatically active protein? Select all options that may be useful.Explain your reasoning.
A.Make the bacteria synthesize protein A in smaller amounts.
B.Dissolve the protein aggregate in urea, then dilute the solution and gradually remove the urea.
C.Treat the insoluble aggregate with a protease.
D.Make the bacteria overproduce chaperone proteins in addition to protein A.
E.Heat the protein aggregate to denature all proteins, then cool the mixture.

You Wish to Produce a Human Enzyme, Protein A, by Introducing