Total nucleic acids are extracted from a culture of yeast cells and are then mixed with resin beads to which the polynucleotide 5′-TTTTTTTTTTTTTTTTTTTTTTTTT-3′ has been covalently attached.After a short incubation, the beads are then extracted from the mixture.When you analyze the cellular nucleic acids that have stuck to the beads, which of the following is most abundant?

Question

Quizplus · Accepted Answer

The polynucleotide 5′-TTTTTTTTTTTTTTTTTTTTTTTTT-3′ is designed to hybridize with poly(A) tails, which are characteristic of eukaryotic mRNA. Therefore, mRNA would be most abundant among the nucleic acids that bind to the beads.

Total Nucleic Acids Are Extracted from a Culture of Yeast