We can estimate the relative mobility of a population of molecules along the surface of a living cell by fluorescently labeling the molecules of interest, bleaching the label in one small area, and then measuring the speed of signal recovery as molecules migrate back into the bleached area.What is this method called? What does the abbreviation stand for?

Question

Quizplus · Accepted Answer

The method described is called Fluorescence Recovery After Photobleaching (FRAP), which involves bleaching a small area of labeled molecules and then measuring the recovery of fluorescence as unbleached molecules move back into the area. Choice A (SDS) is a type of detergent commonly used in protein solubilization, choice B (SPT) stands for Single Particle Tracking which is a different method for studying molecule mobility, and choice C (GFP) stands for Green Fluorescent Protein which is commonly used as a fluorescent label but is not specific to the FRAP method.

We Can Estimate the Relative Mobility of a Population of Molecules