You have prepared lipid vesicles (spherical lipid bilayers) that contain Na+-K+ pumps as the sole membrane protein.All of the Na+-K+ pumps are oriented in such a way that the portion of the molecule that normally faces the cytosol is on the inside of the vesicle and the portion of the molecule that normally faces the extracellular space is on the outside of the vesicle.Assume that each pump transports one Na+ ion in one direction and one K+ ion in the other direction during each pumping cycle (see Figure 12-27 for how the Na+-K+ pump normally functions in the plasma membrane).
Figure 12-27
Predict what would happen in each of the following conditions:
A.The solutions inside and outside the vesicles contain both Na+ and K+ ions but no ATP.
B.The solution outside the vesicles contains both Na+ and K+ ions; the solution inside contains both Na+ and K+ ions and ATP.
C.The solution outside contains Na+; the solution inside contains Na+ and ATP.
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