Consider the in vitro motility assay using purified kinesin and purified polymerized microtubules shown in Figure 17-37.The three panels are images taken at 1-second intervals.In this figure, three microtubules have been numbered to make it easy to identify them.Which of the following statements about this assay is FALSE?
Figure 17-37
A) Kinesin molecules are attached by their tails to a glass slide.
B) The microtubules used in this assay must be polymerized using conditions that stabilize tubule formation or else they would undergo dynamic instability.
C) ATP must be added for this assay to work.
D) Addition of the nonhydrolyzable ATP analog (AMP-PNP) would cause the microtubules to move faster.
Correct Answer:
Verified
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