You engineer yeast cells that express the M cyclin during S phase by replacing the gene regulatory sequences of the M cyclin gene with those of the S cyclin gene.Keeping in mind that yeast cells have one common Cdk that binds to all cyclins, which of the following outcomes is LEAST likely during this experiment?

Question

Quizplus · Accepted Answer

Since the gene regulatory sequences of the M cyclin gene have been replaced with those of the S cyclin gene, the resulting yeast cells will now express M cyclin during S phase. This means that both M cyclin-Cdk and S cyclin-Cdk complexes will be present during S phase, and some substrates that are normally phosphorylated in M phase will now be phosphorylated in S phase. S-Cdk targets will also be phosphorylated during S phase. However, replacing the regulatory sequences of the M cyclin gene with those of the S cyclin gene is unlikely to affect the timing of G₁ cyclin-Cdk activation in G1, so option C is the least likely outcome.

You Engineer Yeast Cells That Express the M Cyclin During