Ligase enzymes are used to covalently bind together pieces of DNA. True or False?

Question

Quizplus · Accepted Answer

Ligase enzymes catalyze the formation of a covalent bond between adjacent 3' hydroxyl and 5' phosphate termini of DNA, resulting in the formation of a phosphodiester linkage. This process is commonly used in molecular biology to join together DNA fragments, such as during recombinant DNA technology or in the creation of gene knockouts.

Ligase Enzymes Are Used to Covalently Bind Together Pieces of DNA