The most long-lasting consequence of the First Crusade was the:

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While A, C, and D are all significant outcomes of the First Crusade, the most long-lasting consequence was the rapid development of Islamic and Christian doctrines of holy war. The Crusades set a precedent for military expeditions based on religious beliefs and solidified the idea that fighting in the name of one's faith was not only acceptable but necessary. This idea continued to shape international relations and warfare for centuries to come. The fall of the Byzantine Empire, while significant, was not a direct consequence of the First Crusade.

The Most Long-Lasting Consequence of the First Crusade Was The