Which is not one of the elements needed to amplify DNA by polymerase chain reaction (PCR) ?
A) Two primers-short polynucleotides that are complementary to the two ends of the segment to be amplified
B) A restriction endonuclease enzyme that cleaves DNA at specific locations
C) The segment of DNA that must be copied
D) A DNA polymerase enzyme that will catalyze the synthesis of a complementary strand of DNA from a template strand
E) Nucleoside triphosphates that serve as the source of the nucleotides A,T,C,and G needed in the synthesis of the new strands of DNA
Correct Answer:
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