In the Sanger,or chain termination,method of DNA sequencing,incorporation of the nucleotides stops because
A) the dideoxynucleotide analogs have no 3ʹ-OH group to continue DNA synthesis.
B) the dideoxynucleotide analogs have no 5ʹ-OH group to continue DNA synthesis.
C) the dideoxynucleotide analogs have no 2ʹ-OH group to continue DNA synthesis.
D) the mixture is heated to 92°C to stop DNA synthesis at specific time intervals.
E) the dideoxynucleotide analogs are in greater concentration than the actual nucleotides.
Correct Answer:
Verified
Q15: The normal gene for hemoglobin was isolated,cloned
Q16: Whole genome or shotgun sequencing requires
A)the creation
Q17: Antibiotic resistance genes are often included on
Q18: Gel electrophoresis separates DNA molecules based on
Q19: DNA extracted from hair,blood,or other bodily fluids
Q21: Which of the following techniques separates proteins
Q22: What new field combines computer science and
Q23: You have discovered a gene that appears
Q24: You wish to investigate proteins from human
Q25: The use of recombinant DNA technology to
Unlock this Answer For Free Now!
View this answer and more for free by performing one of the following actions
Scan the QR code to install the App and get 2 free unlocks
Unlock quizzes for free by uploading documents