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You Have Cloned a Eukaryotic Gene,and Inserted It into a Bacterial

Question 54

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You have cloned a eukaryotic gene,and inserted it into a bacterial system for expression.You discover that the gene product is much longer than should be observed based upon the insert you successfully cloned.What problems would result in a longer cloned sequence? How will you go about investigating your hypothesis? Would your answer change based upon the presence or absence of introns? Why or why not?

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