Total internal reflection (TIRF)microscopy is best used to visualize fluorescently labeled protein activity
A)that is too small to see with regular light microscopy.
B)on the upper surface of a cell.
C)in the Golgi.
D)on the lower surface of a cell.
E)in the nucleus.

Question

Quizplus · Accepted Answer

Total internal reflection fluorescence (TIRF) microscopy is ideal for visualizing fluorescently labeled proteins on the lower surface of a cell, as it selectively illuminates and excites fluorophores in a very thin region close to the glass-cell interface.

Total Internal Reflection (TIRF)microscopy Is Best Used to Visualize Fluorescently