Using genetic engineering techniques,which of the following would you do to a protein that normally is constitutively secreted to make it accumulate in the ER?
A)fuse it to GFP
B)add an amino acid that would get a mannose-6-phosphate group added to its carbohydrate
C)add a KDEL sequence near its C-terminus
D)mutate one of the asparagines that is normally glycosylated to an alanine,which is not glycosylated
E)None of these would cause the protein to accumulate in the ER.

Question

Quizplus · Accepted Answer

Adding a KDEL sequence near its C-terminus is a recognized signal for retaining proteins in the endoplasmic reticulum (ER). This sequence is recognized by ER retention receptors, preventing the protein from being secreted out of the ER.

Using Genetic Engineering Techniques,which of the Following Would You Do