The DNA primers used in polymerase chain reaction (PCR)are

Question

Quizplus · Accepted Answer

The DNA primers used in PCR are short, synthetic strands of DNA that are complementary to the DNA sequences at both ends of the DNA sequence of interest. They provide a starting point for the DNA polymerase to copy the DNA during each cycle of amplification. Therefore, option D is the correct choice. Option A is incorrect because the primers are typically only about 20 nucleotides long, whereas the entire sequence of one strand of the DNA might be thousands to millions of nucleotides long. Option B is incorrect because primers are not produced by restriction enzymes. Option C is incorrect because the primers are not attached to the gene of interest by polymerase – they are added to the reaction mixture as separate molecules.

The DNA Primers Used in Polymerase Chain Reaction (PCR)are