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How Was Ecoli Was Used to Aid in the Cloning of the of the Bioluminescence

Question 21

Multiple Choice

How was E.coli was used to aid in the cloning of the bioluminescence genes from Vibrio fischeri?


A) Gene fragments from V.fischeri were transformed into E.coli and bioluminescent E.coli were identified.
B) Gene fragments from V.fischeri were cloned into plasmids, transformed into E.coli, and then retransformed back into V.fischeri.
C) Gene fragments from E.coli were used to disrupt the bioluminescence genes of V.fischeri by homologous recombination.
D) Transposons from E.coli were used to mutagenize the bioluminescence genes of V.fischeri.

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