If the Tn10 transposon is used to mutagenize a bacterium for the purpose of creating a mutation in the his operon, what would be the most appropriate selection media?
A)rich media that contains all of the amino acids supplemented with tetracycline
B)minimal media supplemented only with histidine
C)minimal media supplemented with tetracycline and histidine
D)minimal media supplemented with tetracycline alone

Question

Quizplus · Accepted Answer

Tn10 transposon carries a tetracycline resistance gene, so selecting on minimal media with tetracycline ensures that only bacteria with the transposon survive. Adding histidine allows for the selection of mutants in the his operon, as only those that cannot synthesize histidine will require it in the media to grow.

If the Tn10 Transposon Is Used to Mutagenize a Bacterium