During PCR, heating of DNA is required to
A) promote attachment of nucleotides to the template.
B) allow DNA ligases to bind fragments together.
C) unwind double-stranded DNA.
D) attach the primer to the template DNA.
E) attach a stop codon.

Question

Quizplus · Accepted Answer

Heating during PCR is used to denature the double-stranded DNA, causing it to unwind into single strands so that primers can anneal and DNA polymerase can synthesize new DNA strands.

During PCR, Heating of DNA Is Required to