A researcher is attempting to use RNAi to investigate the function of citrate synthase, an enzyme that is involved in the Kreb's cycle. If the researcher injected siRNAs complementary to citrate synthase mRNA into cells, what is the most likely outcome that she would observe?
A)Less citrate synthase RNA would be produced.
B)Less citrate synthase protein would be produced.
C)More citrate synthase protein would be produced.
D)More citrate synthase RNA would be produced.

Question

Quizplus · Accepted Answer

siRNAs would lead to the degradation of citrate synthase mRNA, resulting in less translation and thus less citrate synthase protein production.

A Researcher Is Attempting to Use RNAi to Investigate the Function