Various techniques have been developed to isolate strains of bacteria carrying a particular mutation.One of them is, bacteria containing wild-type cells and cells of a desired mutant are grown on a medium lacking the nutrient for which the desired mutants are auxotrophic but containing an antibiotic that only kills growing cells.In this environment,
A)both wild-type and mutant cells are killed.
B)both wild-type and mutant cells survive.
C)wild-type cells survive; mutant cells die.
D)wild-type cells die; mutant cells survive.

Question

Quizplus · Accepted Answer

In this environment, wild-type cells die because they cannot grow without the nutrient and are killed by the antibiotic, while mutant cells survive because they do not grow and are not affected by the antibiotic.

Various Techniques Have Been Developed to Isolate Strains of Bacteria