You Cross an Hfr Strain of Ala⁺ his^- Val⁺ genotype

You cross an Hfr strain of ala⁺ his^- val⁺ genotype with an F^- strain of ala^- his⁺ val^- genotype and wish to recover F^- ala⁺ his⁺val^- recombinants. Which of the following media should you use that will allow the recombinants to grow and form colonies but not allow growth of the two parental strains?

A) Minimal Moderate with histidine and valine but no alanine
B) Minimal Moderate with valine but no alanine or histidine
C) Minimal Moderate with histidine and alanine but no valine
D) Minimal Moderate with no histidine, alanine, or valine
E) Minimal Moderate with alanine but no histidine or valine