Which of the following is NOT true of bisulfite sequencing?
A) Methylated cytosines are unaffected by sodium bisulfite.
B) DNA is treated with sodium bisulfite, which chemically converts unmethylated cytosines to uracil.
C) Unmethylated cytosines are detected as thymine during sequencing.
D) All thymines revealed in DNA sequencing of bisulfite treated DNA were cytosines in the original, untreated DNA.
E) The sequence of genomic DNA treated with sodium bisulfite is compared to the sequence of untreated genomic DNA.

Question

Quizplus · Accepted Answer

In bisulfite sequencing, only unmethylated cytosines are converted to uracil (and then read as thymine), so not all thymines in the sequenced DNA were originally cytosines.

Which of the Following Is NOT True of Bisulfite Sequencing