You obtain six BACs (of known order, shown below) and six sequence-tagged sites (STSs, of unknown order) derived from an unfinished portion of the genome sequence of Monodelphis domestica, the gray short-tailed opposum. Using PCR, you test each BAC for the presence (+) or absence (-) of each of the STSs. You obtain the following results: Construct a physical map that shows the order of the STSs and their approximate locations within each BAC.

Question

You obtain six BACs (of known order, shown below) and six sequence-tagged sites (STSs, of unknown order) derived from an unfinished portion of the genome sequence of Monodelphis domestica, the gray short-tailed opposum.  Using PCR, you test each BAC for the presence (+) or absence (-) of each of the STSs. You obtain the following results:  Construct a physical map that shows the order of the STSs and their approximate locations within each BAC.

You Obtain Six BACs (Of Known Order, Shown Below) and Six