Below are the steps involved in the RNA-seq process. Place these steps in the CORRECT order. 1. Conversion of the RNA to cDNA
2) Fragmentation and preparation of the cDNAs for sequencing
3) Isolation of the RNA molecules of interest from cells
4) Assembly of the sequencing reads into RNA transcripts
5) Sequencing of the cDNA
A) 3, 1, 2, 5, 4
B) 1, 2, 4, 5, 3
C) 3, 4, 1, 2, 5
D) 1, 5, 4, 3, 2
E) 1, 5, 2, 3, 4

Question

Quizplus · Accepted Answer

The correct order of steps in the RNA-seq process is: 3) Isolation of RNA, 1) Conversion to cDNA, 2) Fragmentation and preparation, 5) Sequencing, and 4) Assembly of reads.

Below Are the Steps Involved in the RNA-Seq Process