Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) is a technique wherein proteins are separated in one dimension by a chemical property and in a second dimension by a different property.
-By what property are proteins separated in the second dimension?
A) electrical charge
B) molecular mass
C) posttranslational modification
D) isotope
E) hydrophobic index

Question

Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) is a technique wherein proteins are separated in one dimension by a chemical property and in a second dimension by a different property. 
-By what property are proteins separated in the second dimension?
A) electrical charge
B) molecular mass
C) posttranslational modification
D) isotope
E) hydrophobic index

Quizplus · Accepted Answer

In 2D-PAGE, proteins are first separated by isoelectric focusing based on their isoelectric point (pI) and then by SDS-PAGE based on their molecular mass.

Two-Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) Is a Technique Wherein Proteins