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You identify an RFLP in mice by digesting genomic DNA with HindIII enzyme and radiolabeling a piece of probe DNA. Southern blot analysis shows that the probe detects a 2-kb fragment in one strain of mice and a 4-kb fragment in another strain of mice. The two mice strains are crossed to produce an F1 generation. Two F1 siblings are mated to produce a dozen F2 progeny. You isolate genomic DNA from several F1 and F2 individuals, digest with HindIII, and perform a Southern blot with the same probe.
-How many bands would be seen in the Southern blot of an F2 individual?
A) one
B) two
C) three
D) four
E) one or two
Correct Answer:
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