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A fragment of DNA is cloned into a plasmid with a sequencing primer-binding site. After dideoxy sequencing, the gel pattern shown in this diagram is obtained.
-What was the sequence of the DNA strand that acted as the template in the sequencing reaction?

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A fragment of DNA is cloned into a plasmid with a sequencing primer-binding site. After dideoxy sequencing, the gel pattern shown in this diagram is obtained. 
-What was the sequence of the DNA strand that acted as the template in the sequencing reaction?

Quizplus · Accepted Answer

The correct answer is A because the sequence of the DNA strand that acted as the template in the sequencing reaction is 5' GCTAGCA 3'. This is because the sequencing reaction is a process that uses a DNA polymerase to synthesize a new strand of DNA complementary to the template strand. The new strand is synthesized in the 5' to 3' direction, so the template strand must be in the 3' to 5' direction. The sequence of the template strand is therefore 5' GCTAGCA 3'.

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