The following nucleotide sequence is found in a short stretch of DNA: 5ʹ TGCC 3ʹ
3ʹ ACGG 5ʹ
Suppose a depurination event occurred within the top strand. If this mutant strand were used as a template for replication, what is the MOST likely sequence of the newly synthesized strand?

Question

Quizplus · Accepted Answer

Depurination removes a purine base (A or G) from the DNA. If the G in the top strand is lost, the complementary strand will likely incorporate an A opposite the missing base during replication, resulting in the sequence 3ʹ AAGG 5ʹ.

The Following Nucleotide Sequence Is Found in a Short Stretch