You identify a novel deep-sea vent (DSV) bacteria and wish to explore its molecular biology. You find that you can introduce DNA sequences into the bacteria and it will transcribe and translate those sequences. However, you notice some curious differences between the expected proteins and the actual proteins produced.
a. A 9-bp DNA sequence that in E. coli encodes a short peptide of Met-Trp-Phe instead produces a peptide of Met-Cys-Val-Trp-Gly-Val-Phe in the DSV bacteria. What is the sequence of the coding strand of the short DNA fragment? Propose an explanation for the longer peptide produced by the DSV bacteria.
b. Curiously, a 9-bp sequence that in E. coli produces a peptide of Met-Thr-Asn does not produce any peptide in the DSV bacteria. What is the sequence of the coding strand of this DNA fragment? Propose an explanation for the absence of any peptide.

Question

Quizplus · Accepted Answer

The Answer of You identify a novel deep-sea vent (DSV) bacteria and wish to explore its molecular biology. You find that you can introduce DNA sequences into the bacteria and it will transcribe and translate those sequences. However, you notice some curious differences between the expected proteins and the actual proteins produced.
a. A 9-bp DNA sequence that in E. coli encodes a short peptide of Met-Trp-Phe instead produces a peptide of Met-Cys-Val-Trp-Gly-Val-Phe in the DSV bacteria. What is the sequence of the coding strand of the short DNA fragment? Propose an explanation for the longer peptide produced by the DSV bacteria.
b. Curiously, a 9-bp sequence that in E. coli produces a peptide of Met-Thr-Asn does not produce any peptide in the DSV bacteria. What is the sequence of the coding strand of this DNA fragment? Propose an explanation for the absence of any peptide.

You Identify a Novel Deep-Sea Vent (DSV) Bacteria and Wish