During DNA replication, the synthesis of the new strand requires the addition of a new dNTP to the 3ʹ-OH group of the growing nucleotide strand by DNA polymerase. Which of the following provides the energy needed for this step?
A) the template strand
B) the energy of pentose sugar
C) 5ʹ end of the growing DNA
D) cleavage of two phosphate groups from the dNTP
E) The reaction does not require an energy source.

Question

Quizplus · Accepted Answer

The energy needed for the addition of a new nucleotide during DNA replication is provided by the cleavage of two phosphate groups from the dNTP, releasing pyrophosphate and providing the necessary energy for the formation of the phosphodiester bond.

During DNA Replication, the Synthesis of the New Strand Requires