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When Chromatin from Any Eukaryote Is Digested with Micrococcal Nuclease

Question 59

Multiple Choice

When chromatin from any eukaryote is digested with micrococcal nuclease (an endonuclease) and fractionated using electrophoresis, DNA fragments of approximately 200 base pairs in length are observed. Which of the following statements explain(s) the observation? (Select all that apply.)


A) The 200-base-pair-long DNA fragments represent the approximate length of DNA wrapped around the histone core.
B) The 200-base-pair-long DNA fragment is a characteristic behavior of micrococcal nuclease on any given free DNA strand.
C) The eukaryotic DNA has an enormous number of repetitive sequences, and the nuclease is cleaving certain repetitive sequences, generating these fragments.
D) The result reveals the conserved composition of the nucleosome, which is the repeating unit that makes up chromatin in all eukaryotes.
E) The cleavage occurs at the exposed linker region between adjacent nucleosomes that does not directly interact with the histone core.

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