A researcher inserted DNA fragments from an organism into plasmids and introduced the modified plasmids into bacterial cells. Which of the following methods would be the most efficient means of identifying which clones contain a specific gene of interest?
A) use a microarray to detect a transcript of the gene
B) use a labeled synthetic probe complementary to the gene sequence
C) use electrophoresis to identify plasmids containing an insert of the expected size
D) sequence the DNA of the plasmids from each isolate
E) assay for activity of the gene product

Question

Quizplus · Accepted Answer

Using a labeled synthetic probe complementary to the gene sequence allows for specific and efficient identification of clones containing the gene of interest by hybridization.

A Researcher Inserted DNA Fragments from an Organism into Plasmids