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When FISH Protocols Call for the Use of "Aged" Slides

Question 19

Multiple Choice

When FISH protocols call for the use of "aged" slides of cells, how can this be accomplished?


A) Baking slides at 60° C for 4-16 hours.
B) Soaking slides in pH 7.0 TRIS buffer and 1% ethanol.
C) Washing slides in pH 6.5 normal saline followed by 1% methanol.
D) Freezing slides at - 70° C for 1 week prior to analysis.

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