If cells are not perfectly flat on a prepared slide, the hybridization efficiency in FISH procedures suffers.

Question

Quizplus · Accepted Answer

In Fluorescence in situ Hybridization (FISH) procedures, the efficiency of hybridization can be significantly affected if the cells or tissue sections are not perfectly flat on the slide. This is because uneven surfaces can lead to inconsistent probe access and binding, resulting in variable signal intensities and potentially misleading results.

If Cells Are Not Perfectly Flat on a Prepared Slide