How does one assure that all PCR amplicons are double- stranded?
A) By lengthening the final primer extension step from 1-2 minutes to up to 10 minutes.
B) By adding DNA ligase to the mixture.
C) By holding the reaction mixture at annealing temperature for about 5 minutes at the end of the cycle.
D) By making sure the dNTPs in the mixture are in excess.
Correct Answer:
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Q15: Which of the following is true regarding
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