If the optimal pH for Taq polymerase is about 7.2, why are the reaction buffers manufactured to have a pH of about 8.5?

Question

Quizplus · Accepted Answer

The pH of reaction buffers is adjusted to be higher than the optimal pH for Taq polymerase because pH decreases with an increase in temperature. During PCR, the temperature is significantly higher than room temperature, leading to a decrease in the pH of the reaction mixture. By starting with a higher pH, the buffer ensures that the pH remains closer to the optimal range for Taq polymerase activity throughout the PCR process.

If the Optimal PH for Taq Polymerase Is About 7